ABSTRACT
Simple, sensitive and rapid spectrofluorimetric procedure is described for the determination of two antihypertensive drugs namely, Bisoprolol fumarate [BSF] and Valsartan [VT]. The effect of solvents was investigated. The fluorescence properties of the two cited drugs showed maximum emission intensity in 0.1N H[2]SO[4] at lambda [em] 298 and 415nm for BSF and VT, respectively, when excited at lambda[ex] 227nm. The calibration graphs were rectilinear from 0.08-1.28 and 0.12-1.6 micro g/ml for BSF and VT respectively. The method, was applied to the determination of the two cited drugs in tablets either single or when co-formulated with hydrochlorothiazide [HZ] with % recoveries of 100.03 +/- 0.57 and 99.70 +/- 0.90 for BSF and VT, respectively. Furthermore, the high sensitivity of the proposed method it allowed its application in spiked human plasma with good % recoveries of 99.73 +/- 2.06 for BSF and 99.94 +/- 1.71 for VT